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The primary aim of aeration is to provide oxygen to cells. The oxygen requirements of animal and insect cells are low compared to bacteria, ranging from 0.1 to 1.6 10 8 mg/cell per hour for animal cells to 0.64 to 2.4 10 8 mg/cell per hour for insect cells (Aunins and Henzler, 1993). Furthermore, culture growth in many cell lines (e.g., CHO) is independent of dissolved oxygen across a range of 5 to 100% of air saturation (Oh et al., 1992). For insect cells the optimum value range of dissolved oxygen has been quoted at 40 to 60% of air saturation (Kloppinger et al., 1990). In most cases, it is not a major problem in meeting oxygen requirements. Headspace aeration and silicone tubing aeration is generally suf cient in laboratory, and in some cases, pilot scale equipment. However, as the scale increases, it becomes necessary to sparge air directly into the medium (especially for insect cells under infection conditions). Due to considerations of bubbleinduced cell damage, particularly in the absence of surfactants for cell protection as well as CO2 ventilation and pH control, aeration of cell cultures requires care. After nearly two decades of research, it is now generally accepted that mechanical damage of freely suspended cells is due to bubble hydrodynamics, in particular the bubble-bursting phenomena at the headspace gas liquid interface (Handa et al., 1987; Tramper et al., 1987; Handa-Corrigan et al., 1989; Oh et al., 1989,

Listing 22.10 shows the completed application with a completed version of the custom form component.

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The standard Keyboard Shortcut editor includes four standard sets of shortcuts: BBEdit: Keyboard shortcut set matching those found in BBEdit Dreamweaver 3: This set uses the standard shortcuts found in Dreamweaver 3, supplemented with additional shortcuts for Dreamweaver 4+ features, such as the Assets panel These additional shortcuts are not necessarily the same as those in the Macromedia Standard set HomeSite: Keyboard shortcut set matching those found in HomeSite Macromedia Standard: The default set of shortcuts incorporating common keyboard combinations in Dreamweaver, Fireworks, and Flash To change from one set to another, open the Keyboard Shortcut editor and choose the desired set from the Current Set drop-down list The changes take effect immediately upon closing the dialog box; you don t have to relaunch Dreamweaver The standard sets are locked and cannot be altered you can only customize a copy of one of the standard sets.

Dreamweaver provides all the controls to accomplish this on top of the Keyboard Shortcut editor The four buttons are as follows: Duplicate Set: Copies the current set (standard or custom) and appends the word copy The duplicate set can be fully customized Rename Set: Renames the current shortcut set Export Set as HTML: Saves a list of the current set of keyboard shortcuts in an HTML format that can be viewed or printed in a browser Delete Set: Removes a keyboard shortcut set When Delete Set is chosen, a list of all custom sets is displayed, and any set except the active one may be removed To remove a sole custom set, select any of the standard shortcut sets prior to choosing Delete Set Each command has up to two shortcuts assigned to it.

This facility makes it possible to retain the originally assigned keyboard shortcut and to add a more personal one To create a custom keyboard shortcut set, follow these steps: 1 Choose Edit Keyboard Shortcuts to open the editor Note that the Keyboard Shortcut editor might take a few moments to load your current keyboard shortcuts..

1 Pekurovsky, D, Shindyalov, I N and Bourne, P E (2002) High Throughput Biological Data Processing on Massively Parallel Computers A Case Study of Pairwise Structure Comparison and Alignment Using the Combinatorial Extension (CE) Algorithm Bioinformatics, Submitted 2 Shindyalov, I N and Bourne, P E (2001) CE: A resource to compute and review 3-D protein structure alignments Nucleic Acids Research, 29(1), 228, 229 3 Thomas, M, Mock, S and Boisseau, J (2000) Development of web toolkits for computational science portals: The NPACI hotpage Proc of the Ninth IEEE International Symposium on High Performance Distributed Computing, 2000 4 Foster, I and Kesselman, C (1997) Globus: A metacomputing infrastructure toolkit International Journal of Supercomputer Applications, 11, 115 128 5 Chaitanya Baru, R M, Rajasekar, A and Wan, M (1998) Proc CASCON 98 Conference, Toronto, Canada, 1998 6 Schmidt, M W et al.

The next time you put any of your Web pages on the remote server, be sure to include the dependent files for that page Alternatively, you could put the file (now found in the Connections folder of the site) directly on the server..

The offsetsToNode() function serves as a translator, converting the byte memory offsets retrieved by getSelection() into readable data. For this reason, you often see the following code combination:

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